Solution NMR

Solution NMR structure of the U5-primer binding site (U5-PBS) domain of murine leukemia virus RNA genome bound to the retroviral nucleocapsid protein


Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleocapsid protein p10-Pol Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.38 KDa
Source organism: Murine leukemia virus
Expression system: Escherichia coli
  • Canonical: P03355 (Residues: 479-534; Coverage: 3%)
Gene name: gag-pol
Sequence domains: Zinc knuckle
RNA (68-MER) Chain: B
Molecule details ›
Chain: B
Length: 68 nucleotides
Theoretical weight: 21.89 KDa
Source organism: Murine leukemia virus
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 12%
Refinement method: distance geometry
Chemical shifts: BMR25052  
Expression systems:
  • Escherichia coli
  • Not provided