› 2mkl

Solution NMR

Solution structure of the fourth constant immunoglobulin domain of nurse shark IgNAR

Released:
DOI: 10.2210/pdb2mkl/pdb
PDB entry 2mkl coloured by chain, ensemble of 10 models, front view.
PDB entry 2mkl coloured by chain, ensemble of 10 models, side view.
PDB entry 2mkl coloured by chain, ensemble of 10 models, top view.
Source organism: Ginglymostoma cirratum
Primary publication:
The structural analysis of shark IgNAR antibodies reveals evolutionary principles of immunoglobulins.
Feige MJ, Gräwert MA, Marcinowski M, Hennig J, Behnke J, Ausländer D, Herold EM, Peschek J, Castro CD, Flajnik M, Hendershot LM, Sattler M, Groll M, Buchner J
Proc Natl Acad Sci U S A 111 8155-60 (2014)
PMID: 24830426

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ig-like domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 105 amino acids
Theoretical weight: 11.83 KDa
Source organism: Ginglymostoma cirratum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q90544 (Residues: 454-556; Coverage: 16%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 90%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Camelid and shark single domain antibodies: structural features and therapeutic potential.
Könning et al. (2017)
14 more

2 mentions without citation

The structural analysis of shark IgNAR antibodies reveals evolutionary principles of immunoglobulins.
Feige et al. (2014)
1 more