› 2miq

Solution NMR

Solution NMR Structure of PHD Type 1 Zinc Finger Domain 1 of Lysine-specific Demethylase Lid from Drosophila melanogaster, Northeast Structural Genomics Consortium (NESG) Target FR824J

Released:
DOI: 10.2210/pdb2miq/pdb
PDB entry 2miq coloured by chain, ensemble of 20 models, front view.
PDB entry 2miq coloured by chain, ensemble of 20 models, side view.
PDB entry 2miq coloured by chain, ensemble of 20 models, top view.
Source organism: Drosophila melanogaster
Entry authors: Xu X, Eletsky A, Shastry R, Maglaqui M, Janjua H, Xiao R, Everett JK, Sukumaran DK, Montelione GT, Szyperski T, Northeast Structural Genomics Consortium (NESG), Chaperone-Enabled Studies of Epigenetic Regulation Enzymes (CEBS)

Function and Biology Details

Reaction catalysed: 
(1a) a [histone H3]-N(6),N(6),N(6)-trimethyl-L-lysine(4) + 2-oxoglutarate + O(2) = a [histone H3]-N(6),N(6)-dimethyl-L-lysine(4) + succinate + formaldehyde + CO(2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysine-specific demethylase 5 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.1 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VMJ7 (Residues: 414-504; Coverage: 5%)
Gene names: CG9088, Kdm5, lid
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 94%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

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