PDBe 2mgz

Solution NMR

Solution structure of RBFOX family ASD-1 RRM and SUP-12 RRM in ternary complex with RNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
RNA-binding protein ASD-1 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.59 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: G5EEW7 (Residues: 97-189; Coverage: 23%)
Gene names: CELE_R74.5, R74.5, asd-1
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
SUPpressor Chain: B
Molecule details ›
Chain: B
Length: 105 amino acids
Theoretical weight: 11.61 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: O45189 (Residues: 20-123; Coverage: 42%)
Gene names: CELE_T22B2.4, T22B2.4, sup-12
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*UP*GP*CP*AP*UP*GP*GP*UP*GP*UP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.85 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 72%
Refinement method: simulated annealing
Chemical shifts: BMR19609  
Expression system: Escherichia coli