Solution NMR

Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(SL4)/RsmE(dimer) 2:1 complex


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Translational regulator CsrA1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 70 amino acids
Theoretical weight: 7.85 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
  • Canonical: P0DPC3 (Residues: 1-59; Coverage: 92%)
Gene names: csrA1, rsmE
Sequence domains: Global regulator protein family
SL4(RsmZ) RNA Chains: B, D
Molecule details ›
Chains: B, D
Length: 21 nucleotides
Theoretical weight: 6.77 KDa
Source organism: Pseudomonas fluorescens
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 40%
Refinement method: simulated annealing
Chemical shifts: BMR19548  
Expression systems:
  • Escherichia coli
  • Not provided