PDBe 2m5b

Solution NMR

The NMR structure of the BID-BAK complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BH3-interacting domain death agonist p11 Chain: B
Molecule details ›
Chain: B
Length: 23 amino acids
Theoretical weight: 2.53 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P55957 (Residues: 80-101; Coverage: 11%)
Gene name: BID
Bcl-2 homologous antagonist/killer Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 18.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16611 (Residues: 18-186; Coverage: 80%)
Gene names: BAK, BAK1, BCL2L7, CDN1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Apoptosis Regulator Bcl-x

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 77%
Refinement method: torsion angle dynamics, simulated annealing, molecular dynamics
Chemical shifts: BMR19045  
Expression systems:
  • Not provided
  • Escherichia coli