PDBe 2m2f

Solution NMR

Function and Biology Details

Reaction catalysed:
Narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha). Similarly cleaves other membrane-anchored, cell-surface proteins to 'shed' the extracellular domains.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Disintegrin and metalloproteinase domain-containing protein 17 Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 9.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P78536 (Residues: 581-642; Coverage: 8%)
Gene names: ADAM17, CSVP, TACE
Sequence domains: Membrane-proximal domain, switch, for ADAM17

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 50%
Refinement method: simulated annealing, torsion angle dynamics
Chemical shifts: BMR18912  
Expression system: Escherichia coli