PDBe 2lxs

Solution NMR

Allosteric communication in the KIX domain proceeds through dynamic re-packing of the hydrophobic core

Released:

Function and Biology Details

Reactions catalysed:
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CREB-binding protein Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 10.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92793 (Residues: 587-673; Coverage: 4%)
Gene names: CBP, CREBBP
Sequence domains: KIX domain
Structure domains: Coactivator CBP, KIX domain
MLL cleavage product C180 Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03164 (Residues: 2840-2858; Coverage: 1%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 81%
Refinement method: simulated annealing
Chemical shifts: BMR18694  
Expression system: Escherichia coli