› 2lvs

Solution NMR

NMR solution structure of a CRISPR repeat binding protein

Released:
DOI: 10.2210/pdb2lvs/pdb
PDB entry 2lvs coloured by chain, ensemble of 20 models, front view.
PDB entry 2lvs coloured by chain, ensemble of 20 models, side view.
PDB entry 2lvs coloured by chain, ensemble of 20 models, top view.
Source organism: Hyperthermus butylicus DSM 5456
Primary publication:
Solution properties of the archaeal CRISPR DNA repeat-binding homeodomain protein Cbp2.
Kenchappa CS, Heidarsson PO, Kragelund BB, Garrett RA, Poulsen FM
Nucleic Acids Res 41 3424-35 (2013)
PMID: 23325851

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative uncharacterized protein Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 12.24 KDa
Source organism: Hyperthermus butylicus DSM 5456
Expression system: Escherichia coli
UniProt:
  • Canonical: A2BLH2 (Residues: 1-103; Coverage: 100%)
Gene name: Hbut_0986
Sequence domains:
Structure domains: Homeodomain-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 83%
Refinement method: DGSA-distance geometry simulated annealing
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering.
Wright et al. (2016)
2 more

2 mentions without citation

Solution properties of the archaeal CRISPR DNA repeat-binding homeodomain protein Cbp2.
Kenchappa et al. (2013)
1 more