› 2lva

Solution NMR

NMR solution structure of the N-terminal domain of human USP28, Northeast structural genomics consortium target HT8470A

Released:
DOI: 10.2210/pdb2lva/pdb
PDB entry 2lva coloured by chain, ensemble of 20 models, front view.
PDB entry 2lva coloured by chain, ensemble of 20 models, side view.
PDB entry 2lva coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Lemak A, Yee A, Houliston S, Garcia M, Dhe-Paganon S, Montelione GT, Arrowsmith C, Northeast Structural Genomics Consortium (NESG), Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 28 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96RU2 (Residues: 22-132; Coverage: 10%)
Gene names: KIAA1515, USP28
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 90%
Refinement method: restrained molecular dynamics
Expression system: Escherichia coli

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