Solution NMR

solution structures of BRD4 second bromodomain with NF-kB-K310ac peptide


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription factor p65 Chain: A
Molecule details ›
Chain: A
Length: 13 amino acids
Theoretical weight: 1.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: Q04206 (Residues: 304-316; Coverage: 2%)
Gene names: NFKB3, RELA
Bromodomain-containing protein 4 Chain: B
Molecule details ›
Chain: B
Length: 128 amino acids
Theoretical weight: 14.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O60885 (Residues: 333-460; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 82%
Refinement method: DGSA-distance geometry simulated annealing, torsion angle dynamics
Chemical shifts: BMR18439  
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)