2lsk

Solution NMR

C-terminal domain of human REV1 in complex with DNA-polymerase H (eta)

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA repair protein REV1 Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 11.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBZ9 (Residues: 1158-1251; Coverage: 8%)
Gene names: REV1, REV1L
Sequence domains: DNA repair protein REV1 C-terminal domain
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
DNA polymerase eta Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y253 (Residues: 524-539; Coverage: 2%)
Gene names: POLH, RAD30, RAD30A, XPV

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 76%
Refinement method: simulated annealing
Chemical shifts: BMR18434  
Expression system: Escherichia coli