Solution NMR

NMR structure of FOXO3a transactivation domains (CR2C-CR3) in complex with CBP KIX domain (2l3b conformation)


Function and Biology Details

Reaction catalysed:
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone lysine acetyltransferase CREBBP Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 10.35 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
  • Canonical: P45481 (Residues: 586-672; Coverage: 4%)
Gene names: Cbp, Crebbp
Sequence domains: KIX domain
Structure domains: Coactivator CBP, KIX domain
Forkhead box protein O3 Chain: B
Molecule details ›
Chain: B
Length: 52 amino acids
Theoretical weight: 5.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O43524 (Residues: 460-483; Coverage: 4%)
Gene names: FKHRL1, FOXO3, FOXO3A
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 13%
Refinement method: simulated annealing
Chemical shifts: BMR18315  
Expression system: Escherichia coli