2lnh

Solution NMR

Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Neural Wiskott-Aldrich syndrome protein Chain: A
Molecule details ›
Chain: A
Length: 65 amino acids
Theoretical weight: 7.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00401 (Residues: 207-270; Coverage: 13%)
Gene name: WASL
Structure domains: CRIB domain
Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 1 Chain: B
Molecule details ›
Chain: B
Length: 67 amino acids
Theoretical weight: 7.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHR4 (Residues: 339-402; Coverage: 13%)
Gene names: BAIAP2L1, IRTKS
Sequence domains: Variant SH3 domain
Structure domains: SH3 Domains
Secreted effector protein EspF(U) Chain: C
Molecule details ›
Chain: C
Length: 48 amino acids
Theoretical weight: 5.3 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DJ89 (Residues: 268-314; Coverage: 14%)
Gene names: espF(U), tccP
Structure domains: Helicase, Ruva Protein; domain 3

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: torsion angle dynamics, molecular dynamics
Chemical shifts: BMR18165  
Expression system: Escherichia coli