2lkx

Solution NMR

NMR structure of the homeodomain of Pitx2 in complex with a TAATCC DNA binding site

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Pituitary homeobox 2 Chain: A
Molecule details ›
Chain: A
Length: 68 amino acids
Theoretical weight: 8.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99697 (Residues: 85-144; Coverage: 19%)
Gene names: ARP1, PITX2, RGS, RIEG, RIEG1
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*GP*CP*TP*CP*TP*AP*AP*TP*CP*CP*CP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 3.89 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*GP*GP*GP*GP*AP*TP*TP*AP*GP*AP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4.06 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 43%
Refinement method: molecular dynamics
Chemical shifts: BMR18015  
Expression systems:
  • Escherichia coli
  • Not provided