PDBe 2leb

Solution NMR

Solution structure of human SRSF2 (SC35) RRM in complex with 5'-UCCAGU-3'

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Serine/arginine-rich splicing factor 2 Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01130 (Residues: 1-101; Coverage: 46%)
Gene names: SFRS2, SRSF2
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*UP*CP*CP*AP*GP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 6 nucleotides
Theoretical weight: 1.85 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 68%
Refinement method: simulated annealing
Chemical shifts: BMR17706  
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided