2l2l

Solution NMR

Solution structure of the coiled-coil complex between MBD2 and p66alpha

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcriptional repressor p66-alpha Chain: A
Molecule details ›
Chain: A
Length: 43 amino acids
Theoretical weight: 5.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86YP4 (Residues: 137-178; Coverage: 7%)
Gene name: GATAD2A
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Methyl-CpG-binding domain protein 2 Chain: B
Molecule details ›
Chain: B
Length: 36 amino acids
Theoretical weight: 4.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBB5 (Residues: 360-393; Coverage: 8%)
Gene name: MBD2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: simulated annealing
Chemical shifts: BMR17138  
Expression system: Escherichia coli BL21(DE3)