Solution NMR

NMR Structure of the complex between the Tfb1 subunit of TFIIH and the activation domain of EKLF

Entry authors: Mas C, Di Lello P, Lafrance-Vanasse J, Omichinski JG

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
General transcription and DNA repair factor IIH subunit TFB1 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 12.9 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
  • Canonical: P32776 (Residues: 2-115; Coverage: 18%)
Gene names: D9740.3, TFB1, YDR311W
Sequence domains: TFIIH p62 subunit, N-terminal domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Krueppel-like factor 1 Chain: B
Molecule details ›
Chain: B
Length: 42 amino acids
Theoretical weight: 4.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q13351 (Residues: 51-90; Coverage: 11%)
Gene names: EKLF, KLF1
Sequence domains: Erythroid krueppel-like transcription factor, transactivation 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli