Solution NMR

Solution structure of the non-covalent complex of the ZNF216 A20 domain with ubiquitin

Entry authors: Garner TP, Long JE, Searle MS, Layfield R

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AN1-type zinc finger protein 5 Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 6.54 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
  • Canonical: B5DF11 (Residues: 1-60; Coverage: 28%)
Gene names: Zfand5, Zfp216, Znf216
Sequence domains: A20-like zinc finger
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.57 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
  • Canonical: P0CG63 (Residues: 305-380; Coverage: 20%)
Gene names: SCD2, UBI4, YLL039C
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 23%
Refinement method: Rigid Body Docking, molecular dynamics
Chemical shifts: BMR17024  
Expression system: Escherichia coli