2kxq

Solution NMR

Solution Structure of Smurf2 WW2 and WW3 bound to Smad7 PY motif containing peptide

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase SMURF2 Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 10.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HAU4 (Residues: 250-333; Coverage: 11%)
Gene name: SMURF2
Sequence domains: WW domain
Structure domains: Ubiquitin Ligase Nedd4; Chain: W;
Mothers against decapentaplegic homolog 7 Chain: B
Molecule details ›
Chain: B
Length: 20 amino acids
Theoretical weight: 2.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15105 (Residues: 203-217; Coverage: 4%)
Gene names: MADH7, MADH8, SMAD7

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shifts: BMR16923  
Expression system: Escherichia coli