› 2kvm

Solution NMR

Solution structure of the CBX7 chromodomain in complex with a H3K27me2 peptide

Released:
DOI: 10.2210/pdb2kvm/pdb
PDB entry 2kvm coloured by chain, ensemble of 20 models, front view.
PDB entry 2kvm coloured by chain, ensemble of 20 models, side view.
PDB entry 2kvm coloured by chain, ensemble of 20 models, top view.
Source organism: Mus musculus
Primary publication:
Molecular interplay of the noncoding RNA ANRIL and methylated histone H3 lysine 27 by polycomb CBX7 in transcriptional silencing of INK4a.
Yap KL, Li S, Muñoz-Cabello AM, Raguz S, Zeng L, Mujtaba S, Gil J, Walsh MJ, Zhou MM
Mol Cell 38 662-74 (2010)
PMID: 20541999

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162848 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromobox protein homolog 7 Chain: A
Molecule details ›
Chain: A
Length: 74 amino acids
Theoretical weight: 8.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VDS3 (Residues: 1-71; Coverage: 45%)
Gene names: Cbx7, D15Ertd417e
Sequence domains: Chromo (CHRromatin Organisation MOdifier) domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
histone H3 peptide (residues 15-30) with dimethylated lysine 27 Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.73 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 1 x MLY

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 79%
Refinement method: simulated annealing, torsion angle dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

477 review citations

Genome regulation by long noncoding RNAs.
Rinn et al. (2012)
476 more

1 mention without citation

Molecular interplay of the noncoding RNA ANRIL and methylated histone H3 lysine 27 by polycomb CBX7 in transcriptional silencing of INK4a.
Yap et al. (2010)