Solution NMR

Human NEDD4 3rd WW Domain Complex with Ebola Zaire Virus Matrix Protein VP40 Derived Peptide ILPTAPPEYMEA

Entry authors: Iglesias-Bexiga M, Macias M, Bonet R, Blanco FJ, Cobos ES, Luque I

Function and Biology Details

Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase NEDD4 Chain: A
Molecule details ›
Chain: A
Length: 49 amino acids
Theoretical weight: 5.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P46934 (Residues: 834-878; Coverage: 3%)
Gene names: KIAA0093, NEDD4, NEDD4-1, PIG53
Sequence domains: WW domain
Matrix protein VP40 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.33 KDa
Source organism: HTLV-1 isolate Mel 15
Expression system: Not provided
  • Canonical: Q05128 (Residues: 5-16; Coverage: 4%)
Gene name: VP40

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 54%
Refinement method: DGSA-distance geometry simulated annealing
Chemical shifts: BMR16575  
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided