2kgi

Solution NMR

Solution structure of JARID1A C-terminal PHD finger in complex with H3(1-9)K4me3

Released:

Function and Biology Details

Reaction catalysed:
(1a) a [histone H3]-N(6),N(6),N(6)-trimethyl-L-lysine(4) + 2-oxoglutarate + O(2) = a [histone H3]-N(6),N(6)-dimethyl-L-lysine(4) + succinate + formaldehyde + CO(2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lysine-specific demethylase 5A Chain: A
Molecule details ›
Chain: A
Length: 52 amino acids
Theoretical weight: 5.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29375 (Residues: 1609-1659; Coverage: 3%)
Gene names: JARID1A, KDM5A, RBBP2, RBP2
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
H3(1-9)K4me3 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.11 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: simulated annealing
Chemical shifts: BMR16210  
Expression systems:
  • Escherichia coli
  • Not provided