PDBe 2kek

Solution NMR

Solution structure of a dimer of LAC repressor DNA-binding domain complexed to its natural operator O3

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Lactose operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 62 amino acids
Theoretical weight: 6.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-62; Coverage: 17%)
Gene names: JW0336, b0345, lacI
Sequence domains: Bacterial regulatory proteins, lacI family
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*CP*GP*GP*CP*AP*GP*TP*GP*AP*GP*CP*GP*CP*AP*AP*CP*GP*CP*AP*AP*TP*TP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 7.08 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
DNA (5'-D(P*GP*AP*AP*TP*TP*GP*CP*GP*TP*TP*GP*CP*GP*CP*TP*CP*AP*CP*TP*GP*CP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 7.05 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided