2k42

Solution NMR

Solution Structure of the GTPase Binding Domain of WASP in Complex with EspFU, an EHEC Effector

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin nucleation-promoting factor WAS Chain: A
Molecule details ›
Chain: A
Length: 72 amino acids
Theoretical weight: 8.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42768 (Residues: 242-310; Coverage: 14%)
Gene names: IMD2, WAS
Sequence domains: P21-Rho-binding domain
Structure domains: CRIB domain
ESPFU Chain: B
Molecule details ›
Chain: B
Length: 36 amino acids
Theoretical weight: 4.07 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)