Solution NMR

Mouse Itch 3rd WW domain complex with the Epstein-Barr virus latent membrane protein 2A derived peptide EEPPPPYED


Function and Biology Details

Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase Itchy Chain: A
Molecule details ›
Chain: A
Length: 37 amino acids
Theoretical weight: 4.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
  • Canonical: Q8C863 (Residues: 399-432; Coverage: 4%)
Gene name: Itch
Sequence domains: WW domain
Latent membrane protein 2 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
  • Canonical: P13285 (Residues: 54-62; Coverage: 2%)
Gene name: LMP2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 69%
Refinement method: simulated annealing
Chemical shifts: BMR15153  
Expression systems:
  • Escherichia coli BL21
  • Not provided