X-ray diffraction
2.04Å resolution

Dengue virus 4 NS3 helicase in complex with ssRNA


Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Serine protease NS3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 451 amino acids
Theoretical weight: 51.37 KDa
Source organism: Dengue virus 4 Thailand/0348/1991
Expression system: Escherichia coli
  • Canonical: Q2YHF0 (Residues: 1645-2092; Coverage: 13%)
Sequence domains: Flavivirus DEAD domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases
5'-R(*AP*GP*AP*CP*UP*AP*AP*CP*AP*AP*CP*U)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 nucleotides
Theoretical weight: 3.8 KDa
Source organism: Dengue virus 4 Thailand/0348/1991
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 132.033Å b: 105.497Å c: 72.517Å
α: 90° β: 117.54° γ: 90°
R R work R free
0.199 0.197 0.242
Expression systems:
  • Escherichia coli
  • Not provided