› 2jh2

X-ray diffraction
2.5Å resolution

X-ray crystal structure of a cohesin-like module from Clostridium perfringens

Released:
DOI: 10.2210/pdb2jh2/pdb
PDB entry 2jh2 coloured by chain, front view.
PDB entry 2jh2 coloured by chain, side view.
PDB entry 2jh2 coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Three-dimensional structure of a putative non-cellulosomal cohesin module from a Clostridium perfringens family 84 glycoside hydrolase.
Chitayat S, Gregg K, Adams JJ, Ficko-Blean E, Bayer EA, Boraston AB, Smith SP
J Mol Biol 375 20-8 (2008)
PMID: 17999932

Function and Biology Details

Reaction catalysed: 
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-GlcNAcase NagJ Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 148 amino acids
Theoretical weight: 15.55 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0TR53 (Residues: 764-909; Coverage: 15%)
Gene names: CPF_1442, nagJ
Sequence domains: Cohesin domain
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: R32
Unit cell:
a: 106.776Å b: 106.776Å c: 221.309Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.232 0.295
Expression system: Escherichia coli

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Citations

2 review citations

Insights into cellulosome assembly and dynamics: from dissection to reconstruction of the supramolecular enzyme complex.
Smith et al. (2013)
1 more

4 mentions without citation

Assessing the accuracy of template-based structure prediction metaservers by comparison with structural genomics structures.
Gront et al. (2012)
3 more