PDBe 2j3w

X-ray diffraction
2.1Å resolution

The crystal structure of the bet3-trs31-sedlin complex.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Trafficking protein particle complex subunit 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 142 amino acids
Theoretical weight: 16.71 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CQP2 (Residues: 1-140; Coverage: 100%)
Gene names: Sedl, Trappc2
Sequence domains: Sedlin, N-terminal conserved region
Structure domains: Beta-Lactamase
Trafficking protein particle complex subunit 5 Chains: B, F
Molecule details ›
Chains: B, F
Length: 188 amino acids
Theoretical weight: 21.06 KDa
Source organism: Danio rerio
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6DGL5 (Residues: 1-188; Coverage: 100%)
Gene name: trappc5
Sequence domains: Transport protein particle (TRAPP) component
Structure domains: Trafficking protein particle complex subunit 3
Trafficking protein particle complex subunit 3 Chains: D, E
Molecule details ›
Chains: D, E
Length: 182 amino acids
Theoretical weight: 20.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O55013 (Residues: 1-180; Coverage: 100%)
Gene names: Bet3, Trappc3
Sequence domains: Transport protein particle (TRAPP) component
Structure domains: Trafficking protein particle complex subunit 3

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: C2
Unit cell:
a: 232.211Å b: 63.063Å c: 72.018Å
α: 90° β: 91.72° γ: 90°
R-values:
R R work R free
0.23 0.23 0.276
Expression system: Escherichia coli BL21(DE3)