PDBe 2j3t

X-ray diffraction
2.4Å resolution

The crystal structure of the bet3-trs33-bet5-trs23 complex.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Trafficking protein particle complex subunit 3 Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O55013 (Residues: 1-180; Coverage: 100%)
Gene names: Bet3, Trappc3
Sequence domains: Transport protein particle (TRAPP) component
Trafficking protein particle complex subunit 6A Chain: B
Molecule details ›
Chain: B
Length: 159 amino acids
Theoretical weight: 17.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75865 (Residues: 1-159; Coverage: 100%)
Gene names: HSPC289, TRAPPC6A
Sequence domains: Transport protein particle (TRAPP) component
Trafficking protein particle complex subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 145 amino acids
Theoretical weight: 16.91 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NCF2 (Residues: 1-145; Coverage: 100%)
Gene name: Trappc1
Sequence domains: Sybindin-like family
Structure domains: Beta-Lactamase
Trafficking protein particle complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 219 amino acids
Theoretical weight: 24.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y296 (Residues: 1-219; Coverage: 100%)
Gene names: CGI-104, HSPC172, PTD009, SBDN, TRAPPC4
Sequence domains: Sybindin-like family
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 57.602Å b: 66.336Å c: 200.808Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.249
Expression system: Escherichia coli BL21(DE3)