› 2izx

X-ray diffraction
1.3Å resolution

Molecular Basis of AKAP Specificity for PKA Regulatory Subunits

Released:
DOI: 10.2210/pdb2izx/pdb
PDB entry 2izx coloured by chain, front view.
PDB entry 2izx coloured by chain, side view.
PDB entry 2izx coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of AKAP specificity for PKA regulatory subunits.
Gold MG, Lygren B, Dokurno P, Hoshi N, McConnachie G, Taskén K, Carlson CR, Scott JD, Barford D
Mol Cell 24 383-95 (2006)
PMID: 17081989

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146799 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase type II-alpha regulatory subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 41 amino acids
Theoretical weight: 4.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13861 (Residues: 4-44; Coverage: 10%)
Gene names: PKR2, PRKAR2, PRKAR2A
Sequence domains: Regulatory subunit of type II PKA R-subunit
Structure domains: cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain
AKAP-IS Chain: C
Molecule details ›
Chain: C
Length: 18 amino acids
Theoretical weight: 2.08 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand DTD 2 x DTD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 41.352Å b: 45.251Å c: 56.837Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.194
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

60 review citations

cGMP-dependent protein kinases and cGMP phosphodiesterases in nitric oxide and cGMP action.
Francis et al. (2010)
59 more

12 mentions without citation

Networking with AKAPs: context-dependent regulation of anchored enzymes.
Welch et al. (2010)
11 more