PDBe 2ifd

X-ray diffraction
2Å resolution

Crystal structure of a remote binding site mutant, R492L, of CDC25B Phosphatase catalytic domain

Released:

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
M-phase inducer phosphatase 2 Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 20.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P30305 (Residues: 391-564; Coverage: 30%)
Gene names: CDC25B, CDC25HU2
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 49.957Å b: 71.32Å c: 75.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.212
Expression system: Escherichia coli BL21