2ief

X-ray diffraction
2.6Å resolution

Structure of the cooperative Excisionase (Xis)-DNA complex reveals a micronucleoprotein filament

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Excisionase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 55 amino acids
Theoretical weight: 6.79 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03699 (Residues: 1-55; Coverage: 76%)
Gene name: xis
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2
15-mer DNA Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.62 KDa
Source organism: Escherichia virus Lambda
Expression system: Not provided
19-mer DNA Chain: E
Molecule details ›
Chain: E
Length: 19 nucleotides
Theoretical weight: 5.84 KDa
Source organism: Escherichia virus Lambda
Expression system: Not provided
34-mer DNA Chain: F
Molecule details ›
Chain: F
Length: 34 nucleotides
Theoretical weight: 10.4 KDa
Source organism: Escherichia virus Lambda
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3121
Unit cell:
a: 111.655Å b: 111.655Å c: 76.268Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided