2i06

X-ray diffraction
2.2Å resolution

Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- Locked form

Released:
Source organism: Escherichia coli
Entry authors: Oakley AJ, Mulcair MD, Schaeffer PM, Dixon NE

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA replication terminus site-binding protein Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 35.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P16525 (Residues: 1-309; Coverage: 100%)
Gene names: JW1602, b1610, tau, tus
Sequence domains: DNA replication terminus site-binding protein (Ter protein)
Structure domains:
5'-D(*T*TP*AP*GP*TP*TP*AP*CP*AP*AP*CP*AP*TP*AP*CP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*TP*G*AP*TP*AP*TP*GP*TP*TP*GP*TP*AP*AP*CP*TP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 62.834Å b: 62.834Å c: 252.454Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.28
Expression systems:
  • Escherichia coli
  • Not provided