2hku

X-ray diffraction
2Å resolution

Structural Genomics, the crystal structure of a putative transcriptional regulator from Rhodococcus sp. RHA1

Released:
Source organism: Rhodococcus jostii RHA1
Entry authors: Tan K, Evdokimova E, Kagan O, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 23.31 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q0SB15 (Residues: 1-211; Coverage: 100%)
Gene name: RHA1_ro03468
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2221
Unit cell:
a: 94.4Å b: 123.701Å c: 73.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.259
Expression system: Escherichia coli BL21