2hkq

X-ray diffraction
1.86Å resolution

Crystal structure of the C-terminal domain of human EB1 in complex with the CAP-Gly domain of human Dynactin-1 (p150-Glued)

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Microtubule-associated protein RP/EB family member 1 Chain: A
Molecule details ›
Chain: A
Length: 80 amino acids
Theoretical weight: 9.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15691 (Residues: 191-268; Coverage: 29%)
Gene name: MAPRE1
Sequence domains: EB1-like C-terminal motif
Dynactin subunit 1 Chain: B
Molecule details ›
Chain: B
Length: 97 amino acids
Theoretical weight: 10.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14203 (Residues: 18-111; Coverage: 7%)
Gene name: DCTN1
Sequence domains: CAP-Gly domain
Structure domains: CAP Gly-rich-like domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: C2
Unit cell:
a: 53.156Å b: 79.597Å c: 38.935Å
α: 90° β: 109.29° γ: 90°
R-values:
R R work R free
0.179 0.177 0.217
Expression system: Escherichia coli