2hhw

X-ray diffraction
1.88Å resolution

ddTTP:O6-methyl-guanine pair in the polymerase active site, in the closed conformation

Released:
Primary publication:
The structural basis for the mutagenicity of O(6)-methyl-guanine lesions.
Proc. Natl. Acad. Sci. U.S.A. 103 19701-6 (2006)
PMID: 17179038

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase I Chains: A, D
Molecule details ›
Chains: A, D
Length: 580 amino acids
Theoretical weight: 66.14 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5KWC1 (Residues: 299-878; Coverage: 66%)
Gene names: GK2730, polA
Sequence domains: DNA polymerase family A
Structure domains:
5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(DDG))-3' Chains: B, E
Molecule details ›
Chains: B, E
Length: 9 nucleotides
Theoretical weight: 2.68 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
5'-D(*CP*AP*TP*(6OG)P*CP*GP*AP*GP*TP*CP*AP*GP*G)-3' Chains: C, F
Molecule details ›
Chains: C, F
Length: 13 nucleotides
Theoretical weight: 4.03 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
3 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 93.834Å b: 108.897Å c: 150.111Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.225
Expression systems:
  • Escherichia coli BL21
  • Not provided