PDBe 2hht

X-ray diffraction
2.05Å resolution

C:O6-methyl-guanine pair in the polymerase-2 basepair position

Released:
Primary publication:
The structural basis for the mutagenicity of O(6)-methyl-guanine lesions.
Proc. Natl. Acad. Sci. U.S.A. 103 19701-6 (2006)
PMID: 17179038

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 66.23 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21
UniProt:
  • Best match: Q45458 (Residues: 297-876)
Gene name: polA
5'-D(*GP*CP*GP*AP*TP*CP*AP*GP*CP*TP*CP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
5'-D(*GP*TP*AP*CP*(6OG)P*AP*GP*CP*TP*GP*AP*TP*CP*GP*CP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.94 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 87.864Å b: 93.431Å c: 107.071Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.229
Expression systems:
  • Escherichia coli BL21
  • Not provided