PDBe 2hcb

X-ray diffraction
3.51Å resolution

Structure of AMPPCP-bound DnaA from Aquifex aeolicus

Released:
Source organism: Aquifex aeolicus
Primary publication:
Structural basis for ATP-dependent DnaA assembly and replication-origin remodeling.
Nat. Struct. Mol. Biol. 13 676-83 (2006)
PMID: 16829961

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chromosomal replication initiator protein DnaA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 323 amino acids
Theoretical weight: 37.73 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66659 (Residues: 77-399; Coverage: 81%)
Gene names: aq_322, dnaA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 99.076Å b: 117.714Å c: 200.994Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.266 0.298
Expression system: Escherichia coli