2h1o

X-ray diffraction
3Å resolution

Structure of FitAB bound to IR36 DNA fragment

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Toxin FitB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 143 amino acids
Theoretical weight: 15.92 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5F882 (Residues: 1-138; Coverage: 99%)
Gene names: NGO0907, fitB, vapC
Sequence domains: PIN domain
Structure domains: 5'-nuclease
Antitoxin FitA Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 68 amino acids
Theoretical weight: 7.28 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5F881 (Residues: 2-69; Coverage: 87%)
Gene names: NGO0908, fitA, vapB
Structure domains: Met repressor-like
IR36-strand 1 Chain: U
Molecule details ›
Chain: U
Length: 36 nucleotides
Theoretical weight: 11.17 KDa
IR36-strand 2 Chain: V
Molecule details ›
Chain: V
Length: 36 nucleotides
Theoretical weight: 11.2 KDa

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 75.04Å b: 82.403Å c: 135.503Å
α: 90° β: 94.19° γ: 90°
R-values:
R R work R free
0.269 0.212 0.269
Expression system: Escherichia coli BL21