PDBe 2gzj

X-ray diffraction
1.6Å resolution

Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (D51A)

Source organism: Escherichia coli K-12
Entry authors: Santi PS, Kolade OO, Kuhlmann UC, Kleanthous C, Hemmings AM

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E9 immunity protein Chains: A, E
Molecule details ›
Chains: A, E
Length: 86 amino acids
Theoretical weight: 9.55 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P13479 (Residues: 1-86; Coverage: 100%)
Gene names: ceiE9, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E9 Chains: B, F
Molecule details ›
Chains: B, F
Length: 134 amino acids
Theoretical weight: 15.12 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P09883 (Residues: 450-582; Coverage: 23%)
Gene names: cei, col
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 44.903Å b: 88.248Å c: 52.235Å
α: 90° β: 91.92° γ: 90°
R R work R free
0.187 0.185 0.232
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli