X-ray diffraction
1.6Å resolution

Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. coli DNA Polymerase III

Source organism: Escherichia coli
Entry authors: Park AY, Carr PD, Ollis DL, Dixon NE

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
DNA polymerase III subunit epsilon Chain: A
Molecule details ›
Chain: A
Length: 194 amino acids
Theoretical weight: 21.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P03007 (Residues: 1-191; Coverage: 79%)
Gene names: JW0205, b0215, dnaQ, mutD
Sequence domains: Exonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P41212
Unit cell:
a: 60.099Å b: 60.099Å c: 109.164Å
α: 90° β: 90° γ: 90°
R R work R free
0.162 0.16 0.197
Expression system: Escherichia coli