X-ray diffraction
2.7Å resolution

crystal structure of the wildtype MHV coronavirus non-structural protein nsp15


Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Growth factor-like peptide Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 41.7 KDa
Source organism: Murine hepatitis virus strain A59
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9J3E8 (Residues: 2061-2429; Coverage: 14%)
Gene name: ORF1B
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P6322
Unit cell:
a: 86.435Å b: 86.435Å c: 219.22Å
α: 90° β: 90° γ: 120°
R R work R free
0.203 0.195 0.256
Expression system: Escherichia coli BL21(DE3)