2gpt

X-ray diffraction
1.95Å resolution

Crystal structure of Arabidopsis Dehydroquinate dehydratase-shikimate dehydrogenase in complex with tartrate and shikimate

Released:

Function and Biology Details

Reactions catalysed:
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
3-dehydroquinate = 3-dehydroshikimate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 523 amino acids
Theoretical weight: 57.09 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SQT8 (Residues: 90-603; Coverage: 85%)
Gene names: At3g06350, EMB3004, F24P17.18
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P3221
Unit cell:
a: 96.934Å b: 96.934Å c: 116.157Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.232 0.256
Expression system: Escherichia coli