2gjw

X-ray diffraction
2.85Å resolution

RNA Recognition and Cleavage by an Splicing Endonuclease

Released:
Source organism: Archaeoglobus fulgidus
Primary publication:
RNA recognition and cleavage by a splicing endonuclease.
Science 312 906-10 (2006)
PMID: 16690865

Function and Biology Details

Reaction catalysed:
PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct RNA molecules
Macromolecules (4 distinct):
tRNA-splicing endonuclease Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 313 amino acids
Theoretical weight: 37.11 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O29362 (Residues: 2-305; Coverage: 100%)
Gene names: AF_0900, endA
Sequence domains:
Structure domains:
5'-R(*GP*CP*GP*AP*CP*CP*GP*AP*CP*CP*AP*(DU)P*AP*GP*CP*UP*GP*CP*A)-3' Chains: E, I
Molecule details ›
Chains: E, I
Length: 19 nucleotides
Theoretical weight: 6.06 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided
5'-R(*UP*GP*CP*AP*GP*CP*GP*GP*UP*CP*AP*(A23))-3' Chains: F, J
Molecule details ›
Chains: F, J
Length: 12 nucleotides
Theoretical weight: 3.91 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided
5'-R(*AP*GP*GP*UP*CP*GP*C)-3' Chains: H, L
Molecule details ›
Chains: H, L
Length: 7 nucleotides
Theoretical weight: 2.24 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P21
Unit cell:
a: 89.965Å b: 140.413Å c: 82.048Å
α: 90° β: 111.68° γ: 90°
R-values:
R R work R free
0.25 0.247 0.296
Expression systems:
  • Escherichia coli
  • Not provided