› 2gbk

X-ray diffraction
1.99Å resolution

Crystal Structure of the 9-10 MoaD Insertion Mutant of Ubiquitin

Released:
DOI: 10.2210/pdb2gbk/pdb
PDB entry 2gbk coloured by chain, front view.
PDB entry 2gbk coloured by chain, side view.
PDB entry 2gbk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of ubiquitin insertion mutants support site-specific reflex response to insertions hypothesis.
Ferraro DM, Ferraro DJ, Ramaswamy S, Robertson AD
J Mol Biol 359 390-402 (2006)
PMID: 16647719

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-143327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 83 amino acids
Theoretical weight: 9.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-617, 618-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P1
Unit cell:
a: 30.046Å b: 45.895Å c: 54.382Å
α: 79.91° β: 74.99° γ: 81.33°
R-values:
R R work R free
0.246 0.244 0.287
Expression system: Escherichia coli BL21(DE3)

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Citations

4 citation in other articles

Thermodynamic analysis of an antagonistic folding-unfolding equilibrium between two protein domains.
Cutler et al. (2007)
3 more