2g9n

X-ray diffraction
2.25Å resolution

Structure of the DEAD domain of Human eukaryotic initiation factor 4A, eIF4A

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic initiation factor 4A-I Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60842 (Residues: 20-238; Coverage: 54%)
Gene names: DDX2A, EIF4A, EIF4A1
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 47.799Å b: 78.252Å c: 59.088Å
α: 90° β: 103.43° γ: 90°
R-values:
R R work R free
0.18 0.176 0.257
Expression system: Escherichia coli