X-ray diffraction
2Å resolution

Clostridium difficile Toxin A Fragment Bound to aGal(1,3)bGal(1,4)bGlcNAc

Source organism: Clostridioides difficile
Entry authors: Greco A, Ho JGS, Lin SJ, Palcic MM, Rupnik M, Ng KKS

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Toxin A Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 28.64 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli
  • Canonical: P16154 (Residues: 2456-2710; Coverage: 9%)
Gene names: tcdA, toxA
Structure domains: Cholin Binding

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, GLA
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 38.682Å b: 132.825Å c: 65.917Å
α: 90° β: 100.29° γ: 90°
R R work R free
0.197 0.195 0.236
Expression system: Escherichia coli