2g6n

X-ray diffraction
1.9Å resolution

Strcture of rat nNOS heme domain (BH2 bound) complexed with CO

Released:
Source organism: Rattus norvegicus
Primary publication:
Structural studies of constitutive nitric oxide synthases with diatomic ligands bound.
J. Biol. Inorg. Chem. 11 753-68 (2006)
PMID: 16804678

Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase, brain Chains: A, B
Molecule details ›
Chains: A, B
Length: 420 amino acids
Theoretical weight: 48.61 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29476 (Residues: 299-718; Coverage: 29%)
Gene names: Bnos, Nos1
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 51.97Å b: 111.53Å c: 164.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.262
Expression system: Escherichia coli BL21(DE3)