PDBe 2fnj

X-ray diffraction
1.8Å resolution

Crystal structure of a B30.2/SPRY domain-containing protein GUSTAVUS in complex with Elongin B and Elongin C

Released:
Primary publication:
Structural and functional insights into the B30.2/SPRY domain.
EMBO J. 25 1353-63 (2006)
PMID: 16498413

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein gustavus Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 25.7 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: A1Z6E0 (Residues: 27-251; Coverage: 81%)
Gene names: CG2944, gus
Sequence domains: SPRY domain
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 13.19 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62869 (Residues: 1-118; Coverage: 100%)
Gene names: Elob, Tceb2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P83940 (Residues: 17-112; Coverage: 86%)
Gene names: Eloc, Tceb1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 50.308Å b: 82.489Å c: 118.966Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.351 0.218 0.247
Expression system: Escherichia coli